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tumor tissue array brain glioma tissue microarray (gl2083a)  (U.S Biomax Inc)
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ERβ5 is highly expressed in GBM. Expression of ERβ isoforms was determined by isoform specific RT-qPCR primers using patient-derived GBM cells (A) and established GBM cells (B). ERβ isoforms expression was examined using isoform specific RT-qPCR primers in patient GBM specimens (n=18) (C). A glioma tissue <t>microarray</t> containing control brain (n=16), as well as grade II (n=130), grade III (n=29) and grade IV (n=33) glioma specimens was used to determine ERβ5 expression using immunohistochemistry (D). Quantitation of total score in each grade was done as described in methods section. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001. ns= non significant.
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Average 90 stars, based on 1 article reviews
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ERβ5 is highly expressed in GBM. Expression of ERβ isoforms was determined by isoform specific RT-qPCR primers using patient-derived GBM cells (A) and established GBM cells (B). ERβ isoforms expression was examined using isoform specific RT-qPCR primers in patient GBM specimens (n=18) (C). A glioma tissue microarray containing control brain (n=16), as well as grade II (n=130), grade III (n=29) and grade IV (n=33) glioma specimens was used to determine ERβ5 expression using immunohistochemistry (D). Quantitation of total score in each grade was done as described in methods section. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001. ns= non significant.

Journal: Cancer research

Article Title: Differential effects of estrogen receptor beta isoforms on glioblastoma progression

doi: 10.1158/0008-5472.CAN-17-3470

Figure Lengend Snippet: ERβ5 is highly expressed in GBM. Expression of ERβ isoforms was determined by isoform specific RT-qPCR primers using patient-derived GBM cells (A) and established GBM cells (B). ERβ isoforms expression was examined using isoform specific RT-qPCR primers in patient GBM specimens (n=18) (C). A glioma tissue microarray containing control brain (n=16), as well as grade II (n=130), grade III (n=29) and grade IV (n=33) glioma specimens was used to determine ERβ5 expression using immunohistochemistry (D). Quantitation of total score in each grade was done as described in methods section. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001. ns= non significant.

Article Snippet: Tumor Tissue Array Brain glioma tissue microarray (GL2083a) was purchased from US Biomax, Inc. (Rockville, MD).

Techniques: Expressing, Quantitative RT-PCR, Derivative Assay, Microarray, Immunohistochemistry, Quantitation Assay